Fig. 5
From: BMI1 promotes osteosarcoma proliferation and metastasis by repressing the transcription of SIK1
BMI1 inhibition alters the chromatin status at the BMI1 binding loci in the SIK1 promoter region. A, B ChIP-qPCR of BMI1-associated DNA sequences from the BMI1-binding region of the SIK1 promoter in OS cells. The Gapdh gene was used as a negative control. Sample size = 3. C–H ChIP-qPCR of RING1B-associated, C, D H2AK119ub-associated, E, F and H3K4me3-associated, G,H DNA sequences in the BMI1-binding region of the SIK1 promoter in DMSO-treated or PTC-209-treated OS cells. The y-axis represents enrichment relative to IgG controls. Sample size = 3. A, B ***p < 0.001, compared with IgG-ChIP. C–H **p < 0.01, ***p < 0.001, compared with DMSO