Table 1 RT reaction system
Reagent | Volume(µL) |
|---|---|
5 × RT buffer | 4.0 |
dNTP mixture(l0mM) | 2 |
RT-enhancer | 0.5 |
Super RI | 0.5 |
Oligo(dT)18 | 1.0 |
Rever Tra Ace \、/*/VC 外 | 1.0 |
1.5 µg RNA Sample | X |
RNAase free ddH2O | (ll–X) |
Total volume | 20 µL |
Reagent | Volume(µL) |
|---|---|
5 × RT buffer | 4.0 |
dNTP mixture(l0mM) | 2 |
RT-enhancer | 0.5 |
Super RI | 0.5 |
Oligo(dT)18 | 1.0 |
Rever Tra Ace \、/*/VC 外 | 1.0 |
1.5 µg RNA Sample | X |
RNAase free ddH2O | (ll–X) |
Total volume | 20 µL |