Table 3 RT-PCR reaction system
Component | Volume (pL) | Final Concentration |
|---|---|---|
2.5 × Real Master Mix 20 × SYBR solution | 10 | L × |
Primer#l (10 pmol/µL) | 0.5 | 0.25 µM |
Primer#2 (10 pmol/µL) | 0.5 | 0.25 µM |
|cDNA | 1 | |
ddHzO | 8 | |
Total volume | 20 |
Component | Volume (pL) | Final Concentration |
|---|---|---|
2.5 × Real Master Mix 20 × SYBR solution | 10 | L × |
Primer#l (10 pmol/µL) | 0.5 | 0.25 µM |
Primer#2 (10 pmol/µL) | 0.5 | 0.25 µM |
|cDNA | 1 | |
ddHzO | 8 | |
Total volume | 20 |