Fig. 4

B28 induce cell apoptosis and bioenergetics dysfunction dependent on excessive ROS generation. A PANC-1 cells were pretreated with ROS scavenger NAC followed by B28 addition for another 24 h, total ROS level was measured and analyzed by flow cytometry. B Cell growth curves of vehicle, B28 and B28 plus NAC treated cells. C Colony images and quantitative analysis of vehicle, B28 and B28 + NAC treated PANC-1 cells. D Cell cycle distribution analysis of indicated groups was analyzed by flow cytometry. E Cell apoptosis in vehicle, B28 and B28 + NAC treated PANC-1 cells were measured and analyzed by flow cytometry. F Vehicle, B28 and B28 + NAC treated PANC-1 cells were collected and subjected to western blot analysis with indicated antibodies. G Analysis of mitochondrial membrane potential alteration in vehicle, B28 and B28 + NAC treated PANC-1 cells. H PANC-1 cells were pretreated with NAC followed by B28 addition, cells were fixed and stained with DAPI and Mito-Tracker, fluorescence of mitochondria and nucleus was captured by confocal laser scanning microscope. I PANC-1 cells were cultured with vehicle, B28 or B28 + NAC, collected cell samples were subjected to western blot analysis with indicated antibodies. J, K Overall OCR and ECAR curves of vehicle, B28 and B28 + NAC treated cells. L PANC-1 cells were treated with vehicle, B28 or B28 + NAC for 24 h, cell samples were collected and analyzed by western blot with primary antibodies. Data are shown as Mean ± SD. **p < 0.01, ***p < 0.001, n.s. represents no significance