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Fig. 5 | Cancer Cell International

Fig. 5

From: A synthesized olean-28,13β-lactam targets YTHDF1-GLS1 axis to induce ROS-dependent metabolic crisis and cell death in pancreatic adenocarcinoma

Fig. 5

GLS1 is a potential downstream target of B28. A PANC-1 and CFPAC-1 cells were treated with B28 or supplemented with multiple metabolites, relative cell viability was measured. B Ratio of GSH and GSSG in vehicle and B28 treated cells. C Vehicle and B28 treated PAAD cells were collected and subjected to western blot with indicated antibodies. D Relative cell viability in shCont and GLS1 knockdown PAAD cells. E Ratio of GSH and GSSG in shCont and GLS1 knockdown PAAD cells. F Total ROS level in shCont and GLS1 knockdown PAAD cells. G Expression of GLS1 in shCont, shGLS1#1 and shGLS1#1 + NAC cells. H Cell growth curves of shCont, shGLS1#1 and shGLS1#1 + NAC cells. I, J Overall OCR and ECAR curves in shCont, shGLS1#1 and shGLS1#1 + NAC cells. Data are shown as Mean ± SD. **p < 0.01, ***p < 0.001

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Cancer Cell International

ISSN: 1475-2867

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