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Fig. 6 | Cancer Cell International

Fig. 6

From: A synthesized olean-28,13β-lactam targets YTHDF1-GLS1 axis to induce ROS-dependent metabolic crisis and cell death in pancreatic adenocarcinoma

Fig. 6

B28 regulates GLS1 expression depends on down-regulation of YTHDF1. A YTHDF1 expression in vehicle and B28 treated cell samples. B shCont and YTHDF1 depleted cells were subjected to western blot analysis with indicated antibodies. C Relative cell viability and total ROS level in control and YTHDF1 knockdown PAAD cells. D, E Overall OCR and ECAR curves in control and YTHDF1 depleted cells. F Control and YTHDF1 overexpressed cells were treated with or without B28, relative cell viability was measured by CCK-8 assay. G Control and YTHDF1 overexpressed cells were cultured with or without B28, cell apoptosis were analyzed by flow cytometry. HandI overall OCR and ECAR curves of CMV + vehicle, CMV + B28, YTHDF1 OE + vehicle and YTHDF1 OE + B28, respectively. Data are shown as Mean ± SD. **p < 0.01, ***p < 0.001

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Cancer Cell International

ISSN: 1475-2867

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