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Fig. 1 | Cancer Cell International

Fig. 1

From: Cell type-specific response of colon cancer tumor cell lines to oncolytic HSV-1 virotherapy in hypoxia

Fig. 1

Construction and characterization of the HMGB1-harboring HSV. A and B Schematic diagram of herpes simplex virus showing the regions modified in HSV-HMGB1 and HSV-ble. The parental virus, HSV-ble, was generated by inserting Blecherry expression cassette into the tk locus. HSV/HMGB1 derived from HSV ICP34.5-Blecherry tk- GFP by inserting HMGB1 expression cassette into UL23 (TK) coding region. C PCR analysis of recombinant HSV-HMGB1. PCR was performed using DNA isolated from non-infected HEK293T, HSV-HMGB1 and HSV-ble by hmgb1F and tkF primers. Lane1: the PCR product of non-infected HEK293T; lane2: PCR product of HSV-ble; lane3: PCR product of HSV-HMGB1; Lane M: 1 kb DNA size marker. D Replication of HSV-HMGB1 and HSV-ble in human colorectal cancer cell lines. Cells were infected with HSV-HMGB1 and HSV-ble at MOI 0.1, and the virus yields were determined at 10, 24, 48 and 72 h post-infection

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Cancer Cell International

ISSN: 1475-2867

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