Fig. 2

HMGB1 expression was significantly higher in CRC cell lines infected with HSV-HMGB1 than in uninfected cells. A, C, and E Infected CRC cells with HSV-HMGB1(red) at MOI 0.7. B, D, and F CRC cells stained with anti HMGB1 antibody (green). B, D, and F represent the same microscopic field of (A, C, and E). G Bar graphs show HMGB1 mean fluorescence intensity (MFI) in uninfected and HSV-HMGB1 infected cells. Fluorescence intensities were measured using the ImageJ software. H mRNA expression of Beta–catenin in HT29cells infected with HSV-HMGB1 and HSV-ble under hypoxic and normoxic condition were analysed by reverse transcription-quantitative PCR. Gene expression levels were calculated based on the Delta-Delta Ct relative quantification. Three biological replicates were performed *p < 0.05, **p < 0.01, and ***p < 0.001