Fig. 3

XTT assays with CRC cells under normoxic and hypoxic conditions demonstrating a dose-dependent cytotoxicity 72 h after infection with HSV-HMGB1, and HSV-ble. The relative cell viability was normalised to that of the control (non- infected CRC cells at 72 h). Three independent experiments were performed. For hypoxic conditioned HT29 cell five independent experiments were performed. data are shown as means ± sd. n = 3, *p < 0.05. **p < 0.01, and ***p < 0.001