Fig. 4

Apoptosis analysis by Annexin-V/PI double staining of HCT116, HT29, and SW480 cells infected with HSV-HMGB1 and HSV-ble at MOI 0.1 for 24 h under hypoxic and normoxic condition. Percentages of Annexin-V positive and Annexin-V/PI double-positive cells are shown in the QB4 and QB2 quadrants respectively. B Bar graph representing percentages of apoptotic cells (Annexin-V positive, PI negative) and non-apoptotic dead cells (Annexin-V positive, PI positive) as measured by flow cytometry. Data are shown as means ± s.d. At least two independent experiments were performed., *p < 0.05