Fig. 5

A Immunoblot analysis of HSV-HMGB1 and HSV-ble infected CRC cell lines. HCT116, HT29 and SW480 were infected with HSV-HMGB1 and HSV-ble at MOI 0.1 under hypoxic and normoxic conditions. The cell lines were also treated with 100 mM Trehalose for 24 h. Cells were harvested after 24 h and subjected to immunoblotting using anti-LC3, and anti-Beclin1 antibody. An antibody against β-actin was used to normalize blotted protein levels. Expression of LC3 and Beclin1was quantified using Image J software. The standard deviation was used to assess data dispersion. At least two independent experiments were performed. B LC3 positive cells were preincubated in the presence of 10 mM of 3-methyladenine (3MA) for 20 min then expression of LC3-II formation was investigated by western blotting with β-actin being an internal control. Expression of LC3 and LC3 + 3MA was quantified using Image J software. The standard deviation was used to assess data dispersion. At least two independent experiments were performed.*p < 0.05, **p < 0.01, and ***p < 0.001