Fig. 6

Confocal microscopy analysis of HMGB and p53 localization. 3 × 10⁴ Cells were fixed, permeabilized and stained for HMGB1 or p53 (green). Infected HCT116 and HT29 cells emitting red light. Draq5 was used to visualize the nuclei. A After 24 h, localization of HMGB1 and P53 in HT29 (mutated p53) and HCT116 (wild p53) infected with HSV-HMGB1 at MOI 0.1were analyzed under hypoxic and normoxic condition. B Localization of HMGB1 and P53 in uninfected HT29 and HCT116 cells were also analyzed during hypoxia and normoxia. All data are representative of two experiments. Scale bar: 50 µm