Fig. 3

PKCδ mediates B7-H4 upregulation in CRC cell lines. HCT116 and SW620 cells were treated with various concentrations of TPA (A) or rottlerin (C) for 20 h. B B7-H4 and nuclei in HCT116 cells were stained with a PE-conjugated anti-B7-H4 antibody and DAPI, respectively. D Flow cytometric analysis was used to detect changes in B7-H4 expression in TPA- or rottlerin-treated HCT116 and SW620 cells. CRC cells were treated with TPA or rottlerin for 24 h, and intracellular B7-H4 expression was examined by flow cytometry. E The HCT116 and SW620 cell lines were treated with 1 μM rottlerin and 100 nM TPA for 24 h, and B7-H4 levels were determined by Western blotting. A representative Western blot image is shown in the left panel. Statistical analysis of B7-H4 expression (mean ± SD) from three separate experiments is shown in the right panel. *P < 0.05, **P < 0.01 and ***P < 0.001