Fig. 6

The PKCδ/B7-H4 axis promoted the migration of HCT116 cells. HCT116 cells were treated with a PKCδ-specific siRNA and/or a B7-H4-specific siRNA for 45 h, and B7-H4 protein levels were then determined by Western blot analysis (A). A Transwell assay was performed to examine the constitutive invasion of B7-H4 siRNA/HCT116, PKCδ siRNA/HCT116, PKCδ siRNA + B7-H4 siRNA/HCT116 and con siRNA/HCT116 cells (B). The invasion of B7-H4 siRNA/HCT116 cells was evaluated after the cells were treated with 100 nM TPA for 24 h (C). A wound healing assay was performed to evaluate the effects of PKCδ and B7-H4 on cell migration (D). Experiments were performed in triplicate. *P < 0.05, **P < 0.01 and ***P < 0.001