Fig. 7

A Representative images of lung metastatic nodules developed in mice 54 days after injection of HCT116 cells. The isolated lungs were processed as described in the Materials and Methods section. Scale bar, 5 mm. B, C Representative images of lung metastatic nodules and H&E staining. The red arrowheads indicate metastatic nodules established in the lungs. H&E staining was performed to evaluate lung micrometastases in a pair of mice referred to in A. D Immunohistochemical analysis of p-PKCδ and B7-H4 in the two groups. The data are presented as the mean ± SD values (n = 5 animals/group). *P < 0.05; **P < 0.01