Fig. 6

MiR-146a promoted HepG2 cell proliferation, cycle progression, migration and invasion by targeting FLAP mRNA. a FLAP protein and transcript levels of HepG2 cells in which stably transfected with miR-146a inhibitor transiently transfected with FLAP-NC (control) or FLAP-targeted small interfering RNA (siRNA). b Representative CCK-8 proliferation assays (left) and the cell cycle regulatory proteins expression in HepG2 cells. c Flow cytometry analysis (left) and quantitation (right) in HepG2 cells. d Representative wound-healing scratch assays (left) and quantitation (right) in HepG2 cells. e Representative matrigel invasion assay (left) and adhesion markers expression (right) in HepG2 cells. Data are presented as mean ± SEM. n = 3; **P < 0.01, ***P < 0.001,****P < 0.0001