Fig. 3
Piezo1 promotes HCC invasion, proliferation and metastasis in vitro and in vivo. A Transwell invasion assay was used to detect the invasive capacities of HCCLM3shPiezo1, Hep3BshPiezo1 and the corresponding control cells (n = 6 for each group). B Wound healing assay was used to detect the migratory capacities of Piezo1 interfered HCCLM3 and Hep3B cells(n = 6). C Colony formation assays was used to detect the proliferation of Hep3BshPiezo1, HCCLM3shPiezo1 and the control HCC cells (n = 6 for each group). D Representative images of the cytoskeleton showed that Piezo1 affected the cellular morphology. E Subcutaneous tumors from HCCLM3shPiezo1 and Hep3BshPiezo1 cells and their control cells. Tumor volumes are shown in the right panels (n = 6 for each group). F Orthotopic tumors from HCCLM3shPiezo1 and Hep3BshPiezo1 cells and their control cells. Tumor weight are shown in the right panels (n = 6 for each group). *P < 0.05; **P < 0.01, the difference between groups analyzed using Student’s t test