Fig. 5

PICALM promotes CRC progression through the ERK/MAPK signaling pathway. A The potential mechanism of PICALM in CRC cells was analyzed by KEGG enrichment analysis. The names of enrichment pathways are shown on the left axis. The abscissa is enrichment factor. The high enrichment factor indicates that the enrichment of different proteins in this pathway is significant. B The effects of PICALM overexpression and MAPK inhibitor treatment on HCT 116 and RKO cells proliferation were detected by cell counting experiment. C The effects of PICALM overexpression and MAPK inhibitor treatment on HCT 116 and RKO cells apoptotic using flow cytometry analysis based on Annexin V-APC staining. D The effect of PICALM on the expression of canonical components of the ERK/MAPK signaling pathway was further examined by western blot analysis. The representative images were selected from at least 3 independent experiments. The data was presented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001