Fig. 3

LRRN4 is highly expressed in CRC cell lines and LRRN4 promotes cell proliferation. A Expression of LRRN4 in SW480, Caco2, HCT-116, LoVo and HIEC-6 cells. B LRRN4 expression in Caco2 and SW480 cells with constitutive expression of two different LRRN4 shRNAs (kd1 or kd2), and LRRN4 expression in HCT-116 and LoVo cells constitutively expressing LRRN4 open reading frame (oe). C EdU proliferation assay and quantification in Caco2 and SW480 cells with LRRN4 knockdown and in HCT-116 and LoVo cells overexpressing LRRN4. D Colony formation assay of cell clonal proliferation ability in Caco2 and SW480 cells with knockdown of LRRN4 and in HCT-116 and LoVo cells overexpressing LRRN4. Bars represent the means with SEM from at least three independent experiments