Fig. 4

LRRN4 promotes cell DNA synthesis and inhibits apoptosis in CRC cells. A Representative histogram of the gated cells in the G0/G1, S and G2/M phases and quantitative analysis of the S phase proportion in Caco2 and SW480 cells with LRRN4 knockdown. B Representative histogram of the gated cells in the G0/G1, S and G2/M phases and quantitative analysis of the S phase proportion in HCT-116 and LoVo cells overexpressing LRRN4. C Representative plots of YF®647A-Annexin V flow cytometry and DAPI staining experiments and quantitative analysis of Annexin V-positive Caco2 with LRRN4 knockdown. D Representative plots of YF®647A-Annexin V flow cytometry and DAPI staining experiments and quantitative analysis of Annexin V-positive SW480 with LRRN4 knockdown. E Representative plots of YF®647A-Annexin V flow cytometry and DAPI staining experiments and quantitative analysis of Annexin V-positive HCT-116 and LoVo cells overexpressing LRRN4. F Representative images of Western blot analyses of PCNA, Chk1, p-Chk1, Bcl-2, Bcl-xl, caspase 3 and cleaved caspase 3 (c-caspase3) in CRC cells with knockdown or overexpression of LRRN4. Bars represent the means with SEM from at least three independent experiments