Technique integration of single-cell RNA sequencing with spatially resolved transcriptomics in the tumor microenvironment

Table 1 Key molecular markers of each cell type in the TME

Population	Subtypes	Marker	References
CAFs	–	α-SMA, vimentin, FAP, PDPN, PDGFRα/β, FSP1, DDR2, S100A4, CD10, GPR77	[36,37,38,39,40,41]
MSCs	–	CD105⁺, CD73⁺, CD70⁺, CD13⁺, CD29⁺, CD44⁺, CD10⁺, CD45⁻, CD34⁻, CD14⁻ or CD11b⁻, CD79a⁻, HLA-DR⁻	[42,43,44]
TAMs	M1	CD68, CD11b, CD80, CD86	[45, 46]
TAMs	M2	CD68, CD11b, CD163, CD206	[45, 46]
DCs	cDC1	XCR1, CD45, CADM1, CLEC9A, CD141	[47, 48]
	cDC2	CD45, CD1C, FcεR1A, CD172A
	pDCs	CD45RA, CD123, CD2
Endothelial cells	–	PECAM1, CD31, CD34, CD13, CD29	[49, 50]
CD4⁺ T cells	–	CD3⁺CD4⁺CD8⁻	[51,52,53,54]
	Th1 cells	CXCR3
	Th2 cells	CCR4
	Th17 cells	CCR6
	Th22 cells	CCR10
	T_reg cells	CD4⁺CD25⁺Foxp3⁺
CD8⁺ T cells	-	CD3⁺ CD8⁺CD4⁻
	Tc1 cells	CRCX3, IRF4
	Tc2 cells	CCR4, CRTH2, GATA3
	Tc9 cells	CRCX3, IRF4, IL9, IL10
	Tc17 cells	CCR6, IL23R, IRF4, IL17
MDSCs	PMN-MDSCs	CD11b⁺CD33⁺HLA⁻DR⁻/CD14⁻CD15⁺	[55, 56]
MDSCs	M-MDSCs	CD11b⁺CD33⁺HLA⁻DR⁻/CD14⁺CD15⁻	[55, 56]

CAFs cancer-associated fibroblasts, MSCs mesenchymal stem cells, TAMs tumor-associated macrophages, DCs dendritic cells, cDCs conventional DCs, pDCs plasmacytoid DCs, MDSCs myeloid-derived suppressor cells, PMN-MDSCs granulocyte-like MDSCs, M-MDSCs monocytic MDSCs

ISSN: 1475-2867