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Fig. 6 | Cancer Cell International

Fig. 6

From: Silencing of histone deacetylase 3 suppresses the development of esophageal squamous cell carcinoma through regulation of miR-494-mediated TGIF1

Fig. 6

shRNA-mediated silencing of HDAC3 suppresses tumor growth of ESCC in vivo through the miR-494/TGIF1/TGFβ axis. A White light images showing xenografts in nude mice. B Tumor volume of nude mice. C Tumor weight of nude mice. D Expression of HDAC3 and TGIF1 in tumor tissues of nude mice. E miR-494 expression in tumor tissues. F Expression of the TGFβ signaling pathway-related proteins (Smad2, Smad4 and TGF-βRII) in tumor tissues detected by Western blot analysis, normalized to GAPDH. G Expression of the TGFβ signaling pathway-related proteins (Smad2, Smad4 and TGF-βRII) in tumor tissues detected by Immunohistochemistry. H Expression of Ki67 in tumor tissues of nude mice and the pathological conditions of tumor tissues. n = 6. *p < 0.05 vs. nude mice harboring cells transfected with sh-NC + oe-NC. #p < 0.05 vs. nude mice harboring cells transfected with sh-HDAC3 + oe-NC. Measurement data were expressed as mean ± standard deviation derived from at least 3 independent experiments. Data comparison among multiple groups was conducted by one-way ANOVA, followed by Tukey’s post hoc test. Tumor volume was analyzed by Bonferroni-corrected repeated measures ANOVA

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Cancer Cell International

ISSN: 1475-2867

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