Fig. 1

Expression of miR-129-5p is regulated by histone acetylation. A Expression of microRNAs after HDAC inhibition was determined by microRNA sequencing. HCC and normal liver cell lines were treated with 2 µM SAHA, 35 nM FK228 or ethanol vehicle control for 24 h. Seventeen miRNAs were upregulated (FC ≥ 2) after SAHA and FK228 treatment in all examined HCC cell lines and were hierarchically clustered. B Induction of miR-129-5p expression after HDAC inhibition was validated by quantitative Real-Time PCR. Results were normalized to ethanol controls and values of ethanol-treated control cells were set to 1 to enable a better comparability of the data. **p < 0.01, ***p < 0.001, ****p < 0.0001; two-way ANOVA with Dunnett’s multiple comparisons test