Fig. 3
From: FGFR1 is a potential therapeutic target in neuroblastoma
FGFR1wt and FGFR1N546K proteins localization. A FGFR1 localization in HEK293 overexspressing FGFR1wt or FGFR1N546K protein was analyzed by Image Stream flow Citometry. The representative figures of single cells at 60X magnification are shown above, whereas the values of mean intensity, standard deviation, cells counted and p-value are reported in the table. B Immunostaining of HEK293, SHSY5Y and SKNBE2 transfected with FGFR1wt or FGFR1N546K analyzed by confocal microscopy. C FGFR1 protein levels in both cytosol and nucleus protein fractions from HEK293, SHSY5Y and SKNBE2 transfected cells was evaluated by western blotting. GAPDH, Lamin β and PARP1 protein levels were used as loading controls