Fig. 4

FGFR1^wt and FGFR1^N546K protein overexpression in NB cells. SH5YSY and SKNBE2 cells were transiently transfected with pCMV6-empty vector, pCMV6-FGFR1^wt and pCMV6-FGFR1^N546K. A Total protein extracts were analyzed by western blotting to evaluate the levels of phosphorylated and total FGFR1, STAT3, ERK, and-AKT. The t-GFP and β-Actin protein levels were used as transfection control and loading control, respectively. B Cell viability in FGFR1^wt and FGFR1^N546K overexpressing cells is shown as fold change compared to the control (pCMV6). C Invading cells, D colony number and area were analyzed. mm² = square millimetres. p = p-value