Fig. 1

OS tumor tissues and cell lines exhibit Tspan7 upregulation. A–D The mRNA-level expression of Tspan7 was markedly increased in OS tissues and cell lines relative to corresponding normal controls (OBs and MSCs) in the GSE (14,359, 12,865, 33,383, and 42,352) datasets. E Relative to Mg63 cells, HOS and Saos2 cells exhibited significantly increased Tspan7 mRNA levels in a qRT-PCR assay, whereas this level was decreased in U2OS cells. GAPDH served as a normalization control. Data are means ± SEM from two separate experiments. F Western blotting results showed that Tspan7 in HOS and Saos2 but not U2OS was markedly higher compared with Mg63 cell. Bands were normalized to the β-actin loading control. Data were presented as the means ± SD of two independent experiments. G ROC curves and AUC values for OS based upon the GSE33383 and GSE42352 dataset. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ROC, receiver operating characteristic; AUC area under the curve, SD standard deviation. **P < 0.01, ****P < 0.0001. Student’s t-test