Fig. 2

Tspan7 knockdown suppresses the proliferation of OS cells; Tspan7-associated biological functions based on RNA-seq analysis. A The knockdown of Tspan7 was confirmed via qRT-PCR at 72 h post-transfection in cells transfected with siTspan7 and siNC. B The viability of cells transfected with siTspan7 or siNC was assessed via CCK-8 assay at 72 h post-transfection. C The effect of Tspan7 silencing on HOS cell growth was assessed in a colony formation assay. Statistical results of colony formation numbers normalized to the NC group were presented. D Top DEGs identified following Tspan7 knockdown in HOS cells are arranged in a heatmap, with shNC cells being used for comparison. E The 764 DEGs identified when comparing shTspan7 and shNC groups at an adjusted |log2FoldChange|≥ 1 and P < 0.05 are shown in a volcano plot, including 416 upregulated genes (red dots) and 348 downregulated genes (green dots). F GO analyses exploring the cellular components, molecular functions, and biological processes in which DEGs were enriched were plotted based upon gene number, with darker blue dots indicating more significant enrichment. G KEGG enrichment signaling analysis results. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Student’s t-test. NC negative control, si small interfering RNA, DEGs different expression genes, GO gene ontology, KEGG kyoto encyclopedia of genes and genomes