Fig. 5
Tspan7 complexes with β1 integrin to promote FAK-Src-Ras-ERK1/2 pathway activation. A Co-immunoprecipitation assays revealed interactions between Tspan7 and integrin β1. B Integrin β1 levels were assessed in HOS-shTspan7 and U2OS-Tspan7 cells via western blotting. C Proteins associated with the FAK-Src-Ras-ERK1/2 pathway (FAK, pFAKY397, pFAKY925, Ras, ERK1/2, and pERK1/2) were assessed by western blotting, with β-actin as a loading control. D Quantification of the western blotting results presented in C. E Signaling downstream of Ras in Tspan7-overexpressing U2OS cells treated with Salirasib (50 μM) was assessed via western blotting. F U2OS-Tspan7 cells treated with or without Salirasib (50 μM) were assessed by migration and invasion assays. Representative cell images are shown, and cells were counted. All experiments were repeated two or three times. *P < 0.05, **P < 0.01, ***P < 0.001