Table 1 The primers and siRNA/shRNA sequences used in this study
Name | Sequences | |
|---|---|---|
Tspan7 | Forward | GCTGCATGAACGAAACTGATTG |
Reverse | GGCGGCCACAGTCAGATT | |
GAPDH | Forward | ATGGAAATCCCATCACCATCTT |
Reverse | CGCCCCACTTGATTTTGG | |
Tspan7 siRNAs | ||
siTspan7#1 | Sense | GCAGACUUACAAUGGCAAUTT |
Antisense | AUUGCCAUUGUAAGUCUGCTT | |
siTspan7#2 | Sense | GGUUGUUAUGAUCUGGUAATT |
Antisense | UUACCAGAUCAUAACAACCTT | |
Negative control (siNC) | Sense | UUCUCCGAACGUGUCACGUTT |
Antisense | ACGUGACACGUUCGGAGAATT | |
Tspan7 shRNAs | ||
shTspan7#1 | 5ʹ-3ʹ | GGTTGTTATGATCTGGTAA |
shTspan7#2 | 5ʹ-3ʹ | GCACCTATATCTCCCTTAT |
Negative control (shNC) | 5ʹ-3ʹ | TTCTCCGAACGTGTCACGT |