Fig. 5

Silencing TIMM8A inhibits the proliferation of breast cancer in vitro. A TIMM8A inhibits proliferation of MDA-MB-231 and MCF7 cells by CCK8 assay (**p < 0.01). B TIMM8A decreases proliferating MDA-MB-231 and MCF7 cells by EdU assay (Scar bar = 100 µm). C The mitochondrial membrane potentials were determined by JC-1 assay (Scar bar = 50 µm). D Quantitative analysis of ratio of red/green fluorescent intensity (**p < 0.01, ***p < 0.001). E The effect of TIMM8A on apoptosis of breast cancer cells was analyzed by Western blot. F The ex vivo model was used to evaluate the effect of TIMM8A on proliferation marker Ki67 in breast cancer tissues (***p < 0.001)