Fig. 1
From: Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43
Morphological characterization of cells from ascitic fluid and characterization of the primary ovarian carcinoma of the patient from whose ascitic fluid IPO43 was established. A Cells were collected by cytospins. Smears of cells stained with Giemsa show aggregates and isolated cells with irregular size, abundant cytoplasm and large nuclei, confirming the presence of malignant cells; Immunocytochemical characterization of cells was done using cytokeratin (CK) type I and II—AE1/AE3 (neoplastic cells are positive); Vimentin (neoplastic cells are predominately negative, macrophages and mesothelial cells were positive) and CA125 (neoplastic cells were positive), 100x. B Formalin fixed paraffin-embedded sections of patient tumor ovarian high grade serous carcinoma submitted to surgery and neoadjuvant chemotherapy (after 3rd cycle) stained with hematoxylin and eosin (H&E) and immunohistochemical staining. Tumor cells show positive expression of epithelial marker Cam 5.2 (CK type II). Apical cell membrane staining for CA 125 in tumor cells. Cells exhibited nuclear positive expression for p53, p16, PAX8 and focally with WT1. Tumor cells were negative with vimentin (VIM), calretinin (CALRET) and HNF1β, 100x