Fig. 7

Growth curve of IPO43 cells during 72 h, after Carboplatin and Paclitaxel treatment. Cells were plated onto a 24 well plates and cell counting was determined by microscopy using the trypan blue exclusion method, and cultured in control condition and exposed to Carboplatin 25 μg/ml, Paclitaxel 10 μg/ml or both drugs during 72 h. Prior to any experiment, cells were synchronized under starvation (culture medium without FBS) for eight hours at 37 °C and 5% CO₂