Table 1 Antigens evaluated and antibodies used to characterize IPO43 cell line
From: Establishment and characterization of a novel ovarian high-grade serous carcinoma cell line—IPO43
Antibody, clone catalog number, source | Antigen | Function/location | Dilution | Positive control |
|---|---|---|---|---|
Anti-Cytokeratin clone AE1/AE3, ISO53, Dako | Cytokeratin type I and II | Cytoskeleton intermediate filament | 1:100 | Skin |
Anti-β-catenin, sc-7199, Santa Cruz Biotechnology | β-catenin | Transcription factor and E-cadherin interacting protein | 1:1000 | Colon cancer |
Anti-CA125 clone OC125, 325 M, Cell Marque | Mucin16 (MUC16) (Cancer antigen 125) | Glycoprotein | 1:150 | Ovarian cancer |
Anti-cytokeratin clone Cam 5.2, 349205, BD Biosciences | Cytokeratin type II | Cytoskeleton | 1:100 | Appendix |
Anti-Calretinin clone DAK-Calret1, M7245, Dako | Calretinin | Calcium-binding protein | Predilute | Mesothelioma |
Anti-CK18 conjugated 488 F4772, Sigma-Aldrich | Cytokeratin type I | Cytoskeleton | 1:100 | Appendix |
Anti-Collagen IV Ab6586, Abcam | Alpha 1 and 2 chains type IV collagen | Basement membrane | 1:10 | Appendix |
Anti-E-cadherin 610181, BD Biosciences | E-cadherin | Adherens junction protein | 1:80 | Breast cancer |
Anti-F-actin, 488, Thermo Fisher | Actin in the filamentous form | Cytoskeleton | 1:100 | Appendix |
Anti-HNF1β HPA002083, Sigma-Aldrich | Hepatocyte nuclear factor 1β (HNF1β) | Transcription factor | 1:1000 | Kidney |
Anti-Ki-67 ab16667, Abcam | Nuclear protein in G1, S, M and G2 phase cell cycle | Cell proliferation | 1:150 | Tonsil |
Anti-CINtec p16, clone E6H4, 725-4713, Ventana Medical System | p16INK4a protein | Transcription factor cell cycle regulator | Predilute | Cervical cancer |
Anti-p53 clone DO7, 453 M, Cell Marque | p53 | Transcription factor cell cycle regulator | 1:150 | Colon cancer |
Anti-Vimentin, clone 3B4, M7020 Dako and V6389, Sigma Aldrich | Vimentin | Cytoskeleton, intermediate filament type III | 1:150 | Appendix |
Anti-PAX8, clone MRQ-50, 760-4618, Ventana Medical System | PAX8 | Transcription factor | Predilute | Fallopian tube |
Anti-WT1, clone 6F-H2, M3561, Dako | Wilm’s tumor 1 factor | Transcription factor | 1:200 | Ovarian cancer |