Fig. 6

IC2 induces apoptosis of MCF-7 cells through the mitochondrial pathway. MCF-7 cells were treated with three concentrations of IC2 for 24 h. A Representative images of JC-1 staining at ×100 magnification in MCF-7 cells. B Flow cytometry was used to detect the deviation of red and green fluorescence in JC-1-stained cells. C Quantification of the ratio of red and green fluorescence in B. D Flow cytometry was used to detect intracellular ROS. E Quantification of the level of ROS production compared to the control in D. Data are presented as the mean ± SEM of three independent experiments (n = 3). *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control