Fig. 2

TMED3 depletion restrains cell proliferation and migration but promotes cell apoptosis. A The comparison in cell proliferation of the experimental groups (shCtrl vs. shTMED3) of HEC-1-B and ishikawa cell lines resulted by Celigo cell counting assay. B The cell cycle detected by FACS and the comparison in cell percentages of three cell cycle stages in the experimental groups (shCtrl vs. shTMED3) of both cell lines. C The difference in the percentage of cell apoptosis in the cell groups (shCtrl vs. shTMED3) of both cell lines. The red-R-fluorescence on the x-axis in the images represented the percentage of apoptotic cells. The y-axis being marked Green-B fluorescence represented the fluorescence signal of the GFP label on the lentivirus used to transfect HEC-1-B and ishikawa cells, which ensured that the detected cells were all successfully transfected cells. D The changes in cell migration ability after scratching the wound in the experimental groups (shCtrl vs. shTMED3) of both cell lines. E The representative photographs (×200) and the comparison in cell migration derived from Transwell assay in cell groups (shCtrl vs. shTMED3) of both cell lines. The left and right panels are experimental results for HEC-1-B cells and ishikawa cells, respectively. The scale bar is 100 μm long. *P < 0.05, **P < 0.01, ***P < 0.001