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Fig. 2 | Cancer Cell International

Fig. 2

From: Nanobodies; new molecular instruments with special specifications for targeting, diagnosis and treatment of triple-negative breast cancer

Fig. 2

Nanobody production procedure. A camel was immunized with a desired antigen. After lymphocyte separation, mRNA isolation and cDNA synthesis were performed. The variable fragment of heavy-chain antibodies (HCAbs) was amplified by PCR and cloned into the phagemid vector. The variable fragment antibody (VHH) was displayed on the surface of an M13 phage. After panning and selection of colonies with the maximum affinity, soluble VHH was expressed in E. coli

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Cancer Cell International

ISSN: 1475-2867

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