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Fig. 2 | Cancer Cell International

Fig. 2

From: Nectin-4 promotes osteosarcoma progression and metastasis through activating PI3K/AKT/NF-κB signaling by down-regulation of miR-520c-3p

Fig. 2

The effect of Nectin-4 on human OS cells proliferation. A, B The expression of Nectin-4 in MG63 and U2OS cells infected with vector negative control (Vector-NC) or Nectin-4 overexpression (Nectin-4-OE) lentivirus verified by RT-qPCR analysis and Western blotting assay, respectively. C, D The effectiveness of shNectin-4#1, #2, and #3 at mRNA level in 143B cells verified by RT-qPCR and Western blotting, respectively. E, F The cell proliferation curve of MG63 and U2OS cells infected with Vector-NC and Nectin-4-OE lentivirus via CCK-8 assay. G The cell proliferation curve of 143B cells in shCtrl group and shNectin-4#2 group using CCK-8 assay. H Colony formation of MG63, U2OS cells (infected with Vector-NC or Nectin-4-OE lentivirus), and 143B cells (infected with shCtrl or shNectin-4#2 lentivirus). Each assay was repeated at least three times. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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