Fig. 4

Nectin-4 modulates EMT and migration potency via PI3K/AKT/NF-κB signal pathway. A The influences of Nectin-4 on the expression of EMT-related markers in 143B, MG63, and U2OS cell lines (infected with Nectin-4-OE, Vector-NC, shCtrl, and shNectin-4#2 Lentivirus, respectively) by RT-qPCR. B The effects of Nectin-4 overexpression or knockdown on the levels of EMT-related markers in cells treated as above by Western blotting. C The effects of Nectin-4 overexpression or knockdown on the levels of PI3K/AKT/NF-κB pathway-related markers in cells treated as above by Western blotting. D The protein expression levels of EMT-related and PI3K/AKT pathway-related markers in Nectin-4-OE U2OS and MG63 cell lines treated with DMSO and LY294002 (PI3K inhibitor). E The protein expression levels of EMT-related and PI3K/AKT pathway-related markers in Nectin-4-OE U2OS and MG63 cell lines treated with DMSO and PDTC (NF-κB inhibitor). F The migration and invasion capacity in Nectin-4-OE U2OS and MG63 cell lines treated with DMSO and LY294002 (scale bars 500 μm, magnifications of 200 ×). The concentration of both LY294002 and PDTC was 10 mM, and dissolved in DMSO. Each assay was repeated for at least three times. ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001