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Fig. 5 | Cancer Cell International

Fig. 5

From: Nectin-4 promotes osteosarcoma progression and metastasis through activating PI3K/AKT/NF-κB signaling by down-regulation of miR-520c-3p

Fig. 5

MiR-520c-3p is a downstream regulator that targets AKT1 and p65. A, B The potential miRNA targeting AKT1 or P65 were predicted by six online bioinformatics analysis software databases, including TargetScan, miRanda, microT, miRmap, RNA22, and PITA. C The miRNAs concurrent targeting AKT1 and p65 were generated by taking the intersection of the two miRNAs clusters using Venn diagrams. D The evaluation of these selected miRNAs expression levels was detected by using RT-qPCR in stable Nectin-4 knockdown 143B cells. E The Spearman′s correlation analysis was performed to discuss the relation between Nectin-4 and miR-520c-3p expression in OS tissues. F The differential expression of miR-520c-3p between primary and metastatic OS samples in the TARGET database. Each assay was repeated at least three times. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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