Fig. 7

The effect of Nectin-4 knockdown on OS cells tumorigenesis and lung metastatic in vivo. A The Subcutaneous transplantation was successfully constructed after being injected with shNectin-4#2 or shCtrl 143B cells. The mice were sacrificed for tumor harvesting after 24 days and tumor image. B The tumor growth curves were plotted in the shNectin-4#2 group and shCtrl group. C The weight of tumors xenografts in the shNectin-4#2 group and the shCtrl group. D The protein expression of ZO-1, Vimentin, Ki67, Nectin-4 in the shNectin-4#2 group and the shCtrl group by using a Western blotting assay. E Hematoxylin–eosin (HE) staining and IHC staining for Ki67, Nectin-4, ZO-1, and Vimentin in shNectin-4#2 group and shCtrl group (scale bars 100 μm, magnifications of 200 ×). (F) Representative images of the lung metastasis model in the shNectin-4#2 group and shCtrl group. G The incidence of mice with lung metastasis in the shNectin-4#2 group and shCtrl group was statistically analyzed. The red arrow represented the lung tissue surface metastases. ns, no significance; *P < 0.05; **P < 0.01; ***P < 0.001