Fig. 3

LY6G6D is epigenetically regulated in mucinous and classical adenocarcinoma. A Up panel, representative IHC pictures of LY6G6D staining in Mucinous and non-Mucinous CRC. Down, LY6G6D and MMR repair immunohistochemical profile in mucinous and classical adenocarcinoma in our CRC database. Up right, stromal quantification of LY6G6D + cells in mucinous versus non-mucinous CRC. B Heatmaps of differentially methylated genes related to Mucinous gene signature in COAD and READ dataset from TCGA. DNA methylation levels are expressed as beta value ranging from 0 (unmethylated, blue) to 1 (fully methylated, purple). C The boxplots show promoter methylation levels of LY6G6D in Mucinous and non-Mucinous CRC in COAD and READ dataset. p-values from t test Welch-corrected are shown. D DNA Methylation profile at the transcription start site (TSS) and gene body of the LY6G6D locus in normal colonic mucosa, low grade and high-grade adenomas, data extrapolated from GSE68060 database