Fig. 7

Glycolysis in WT and GEM-R cells and effect of miR-143 on gem-resistance in vitro. Note: Data are represented as mean ± SD. All experiments were performed in triplicate and repeated independently three times. A CCK8 assays were utilized to evaluate cell viability of GEM-R cells and their parental ones with or without glucose intervention. B RT-PCR showed higher miR-143 level in miR-143 induced GEM-R cells compared with control group. C&D. RT-PCR and Western blot were performed to measure HK2 expression from mRNA and protein levels. Grayscale value ratio = The area of the target protein band/The area of the endogenous reference protein band. E. PET/CT scan was performed to assess the cellular uptake of ¹⁸F-FDG in WT cells, GEM-R cells and GEM-R cells transfected with miR-143. F. Glucose assay kit was used to test glucose consumption among three types of cells