Fig. 8
Efficacy of hENT1 adjuvant with miR-143 on gem-resistance in vitro. Note: Data are represented as mean ± SD. All experiments were performed in triplicate and repeated independently three times. A CCK8 assay was used to evaluated IC50 of GEM-R cells, GEM-R cells transfected with miR-143 and GEM-R-hENT1 cells induced with miR-143 when treated with gradient concentration of GEM. B Percentage of cell apoptosis of GEM-R cells, GEM-R cells transfected with miR-143 and GEM-R-hENT1 cells induced with miR-143 was evaluated by detection of flow cytometry with Annexin-V FITC/PI Apoptosis Detection Kit. Flow cytometric: Annexin V-/P- represented normal cells; Annexin V-/P + represented necrotic cells; Annexin V + /P + represented late apoptotic cells; Annexin V + /P- represented early apoptotic cells