Fig. 2

TRIM36 represses HCC cellular proliferation, migration, and invasion. a Western blotting analysis showing TRIM36 expression level in LO2, HCCLM3, Huh7, HepG2, PLC/PRF/5, and MHCC-97H cell lines. b Left panel of Western blotting analysis showing TRIM36 expression level in HCCLM3 and Huh7 transfected with si-NC, si-TRIM36#1 or si-TRIM36#2, and right panel of Western blotting analysis showing TRIM36 expression level in HCCLM3 and Huh7 transduced with vector or TRIM36. Bar graph showing TRIM36 expression in indicated groups. c Graph showing the proliferation ability measure by Cell counting kit-8 (CCK-8) in HCCLM3 and Huh7 cells transfected with si-NC or si-TRIM36 and HCCLM3 and Huh7 cells transduced with vector or TRIM36 at different timepoints (0 day, 1 day, 2 day, 3 day, 4 day, 5 day and 6 day). d colony formation images in HCCLM3 and Huh7 cells transfected with si-NC or si-TRIM36 and cells transduced with vector or TRIM36. Bar graph showing colony numbers in indicated groups. e Transwell migration and invasion assay showing the migration and invasion ability in HCCLM3 and Huh7 cells transfected with si-NC or si-TRIM36 and cells transduced with vector or TRIM36. Bar graph showing relative migration and invasion in indicated groups. Scale bars, 100 μm. P value was calculated using two-tailed unpaired Student’s t-test within two groups, and one-way ANOVA was used among three groups, error bars are means ± SD, n = 3 independent repeats. *P < 0.05, **P < 0.01, ***P < 0.001