Fig. 5

TRIM36 inhibits Wnt/β-catenin pathway. a, c Western blotting analysis showing TRIM36 and active β-catenin expression in HCC and adjacent non-tumor tissues, p value was calculated using two-tailed paired Student’s t-test, n = 4 independent repeats. b Left panel showing the hematoxylin–eosin staining of HCC and adjacent non-tumor tissues, the remaining set showing immunohistochemistry image of TRIM36, β-catenin, cleaved caspase-3, and Ki-67 in HCC and adjacent non-tumor tissues. Scale bars, 100 μm. The upper panel was 100 × magnification, the lower panel was 200 × magnification. d qRT–PCR showing the expression level of c-Jun and β-catenin mRNA in HCCLM3 and Huh7 transfected with si-NC, si-TRIM36, and cells transduced with vector or TRIM36. e Western blotting analysis showing expression level of CK1, GSK3β, p- GSK3β, c-Jun in HCCLM3 and Huh7 cells transfected with si-NC or si-TRIM36 and cells transduced with vector or TRIM36. Bar graph showing the relative protein expression in indicated groups. P value was calculated using two-tailed unpaired Student’s t-test, error bars are means ± SD, n = 3 independent repeats. *P < 0.05, **P < 0.01, ***P < 0.001