Fig. 6

TRIM36 regulates cellular proliferation, invasion, and migration through the Wnt/β-catenin pathway. a Western blotting analysis showing expression level of TRIM36, MMP9, cyclinD1, total β-catenin, total c-Jun and expression level of nuclear c-jun and nuclear β-catenin in HCCLM3 and Huh7 cells transfected with si-NC, si β-catenin, si-TRIM36 and cells co-transfected with si-β-catenin and si-TRIM36. Bar graph showing the relative protein expression in indicated groups. b Transwell migration and invasion assay showing the migration and invasion ability in HCCLM3 and Huh7 cells transfected with si-NC, si-TRIM36 and co-transfected with si-β-catenin and si-TRIM36. Bar graph showing migration of invasion ability in indicated groups. Scale bars, 100 μm. c Graph showing the proliferation ability measure by Cell counting kit-8 (CCK-8) in HCCLM3 and Huh7 cells transfected with si-NC, si-TRIM36 and co-transfected with si-β-catenin and si-TRIM36 at different timepoints (0 day, 1 day, 2 day, 3 day, 4 day, 5 day and 6 day). d Western blotting analysis showing expression level of TRIM36, active β-catenin and c-Jun expression level in HCCLM3 and Huh7 cells treated with control, WNT3a (100 ng/mL), TRIM36 overexpression and cells co-treated with WNT3a (100 ng/mL) and TRIM36 overexpression. Bar graph showing the relative protein expression in indicated groups. P value was calculated using one-way ANOVA. *P < 0.05 vs NC group, **P < 0.01 vs NC group, ***P < 0.001 vs NC group; #P < 0.05 vs si-TRIM36 group or TRIM36, ##P < 0.01 vs si-TRIM36 group or TRIM36, ###P < 0.001 vs si-TRIM36 or TRIM36 group