Fig. 4

Biological pathways and immunological characteristics of TMSB10 in glioma. A Spearman correlation analysis of TMSB10 and classical signaling pathways in three glioma cohorts. Red indicates positive correlations, and the darkness of color is proportional to the correlation coefficient. The size of the circle represents the statistical P value, with larger circles representing greater statistical significance. Correlation between TMSB10 expression and B the infiltration of 28 tumor-associated TME cells calculated with the ssGSEA algorithm and C immunomodulators. The color indicates the correlation coefficient. The asterisks indicate a statistically significant p value calculated using Spearman correlation analysis. D GSEA showing the (left) classical cancer-promoting pathways and (right) immune-related pathways in the high TMSB10 expression group. E Dot plot of the log2FC (mRNA expression) versus the log2FC (protein expression), showing a positive correlation between the overall mRNA and protein expression level (Pearson’s r = 0.6527) and the distribution of genes with significant changes in both the mRNA expression (|FC| > 2, P < 0.05) and corresponding protein expression (|FC| > 1.2, P < 0.05) in the high TMSB10 expression group compared with the low TMSB10 expression group. F GO BP enrichment analysis of 93 genes that were significantly upregulated at both the mRNA and protein levels. The statistical significance is shown as P < 0.05; **P < 0.01; ***P < 0.001