Fig. 6

TMSB10 promotes the proliferation, migration and invasion of glioma cells in vitro and in vivo. A The mRNA expression level of TMSB10 increased with tumor grade in the Qilu dataset. B ROC curve showing that the expression level of TMSB10 had a high AUC in predicting the grade of glioma in the Qilu dataset. C IHC analysis showing TMSB10 protein expression in normal brain tissues (NBTs) and glioma tissues with different WHO grades. Histogram representing statistical data of IHC. The FTO-positive ratio was defined as the ratio of the FTO-positive area to the total area; n = 3. D Correlations between TMSB10 and the enrichment scores of (left) cancer hallmark pathways and (right) immune cells in the Qilu cohort. CCK-8 assays showing the proliferation ability of GBM cells transfected with (E) sh-NC or sh-TMSB10 and (F) ov-NC or ov-TMSB10; n = 3. G Representative Transwell migration and invasion assays showing the migration and invasion ability of GBM cells transfected with (G) sh-NC or sh-TMSB10 and (H) ov-NC or ov-TMSB10; scale bar, 200 μm. The quantification histogram represents the relative cell numbers. Data represent the mean ± SD from at least three independent experiments. I Representative tumor sphere formation images of GSCs transfected with sh-NC or sh-TMSB10; scale bar, 100 μm. The quantification histogram represents the average sphere diameter. Data represent the mean ± SD from at least three independent experiments. J Bioluminescent image showing the tumor size of mice implanted with luciferase-labeled U87MG cells expressing sh-TMSB10 or sh-NC at the indicated times. The quantification histogram represents the bioluminescent flux. Data represent the mean ± SD; n = 5 for each group. K Kaplan–Meier survival curves for mice implanted with luciferase-labeled U87MG cells expressing sh-TMSB10 or sh-NC. Log-rank analysis was used; n = 5 for each group. L Representative CD44 and KI67 immunohistochemistry images for a subgroup of animals sacrificed simultaneously in each group; n = 5 for each group, scale bar, 20 μm. M Representative H&E staining images for a subgroup of animals sacrificed simultaneously in each group; n = 5 for each group, scale bar, 10 μm. All data are presented as the mean ± SD. The statistical significance is shown as *P < 0.05; **P < 0.01; ****P < 0.0001