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Fig. 3 | Cancer Cell International

Fig. 3

From: METTL3 suppresses anlotinib sensitivity by regulating m6A modification of FGFR3 in oral squamous cell carcinoma

Fig. 3

METTL3 regulates FGFR3 mRNA m6A modification and inhibits FGFR3 mRNA stability. A The METTL3 protein level and m6A level of RNA were detected by western blotting or dot blot in METTL3-knockdown OSCC cells (SCC9 and SCC25). B Representative m6A modification of FGFR3 in OSCC by MeRIP-seq (data from our previous study).C MeRIP-qPCR showed that relative FGFR3 m6A level was significantly decreased after METTL3 knockdown in SCC9 and SCC25 cells. D The FGFR3 protein and mRNA levels, and p-FGFR3 protein levels were significantly increased in METTL3 knockdown OSCC cells (SCC9 and SCC25). E FGFR3 mRNA stability was significantly decreased in METTL3-knockdown OSCC cells after treated with actinomycin D. F. FGFR protein stability was no significant changes between control cells and METTL3-knockdown OSCC cells by cycloheximide assay. Quantification of the protein optical density by ImageJ. *P < 0.05; **P < 0.01; ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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