Table 1 The information of methylation and qRT-PCR primers
Genes | Accession number | Sequence 5′ → 3′ | Start | Tm (°C) | Product size (bp) | No. CpG |
|---|---|---|---|---|---|---|
Methylation primers | ||||||
 NIS | NG_012930.1 | F:GTGATTAGGGGATTATAGTGTATGG | − 775 | 57.13 | 210 | 7 |
R:TAAATTACAAATTTATTAAACTCCC | − 988 | 52.69 | ||||
qRT-PCR primers | ||||||
 NIS | NM_000453.3 | F:CCCAGACCAGTACATGCCTC | – | 59.82 | 86 | – |
R:TGTAAGCACAGGCCAGGAA | 58.85 | |||||
 Β-actin | NM_001101.5 | F:GATCAAGATCATTGCTCCTCCT | – | 57.65 | 108 | – |
R:TACTCCTGCTTGCTGATCCA | 58.43 | |||||